With Operator Assistance: $151.19/hour (internal rate) or $266.70 (external rate) Self-Operated: $56.55/hour (internal rate) or $99.76/hour (external rate) Location: Room S252, Genentech Hall, Mission Bay CampusĬontact: For operator and technical assistance on this cell sorter, please contact Sarah Elmes at. The collection optics consist of octagon- and trigon-shaped collection devices, and a total of up to 18 independent signals can be acquired at one time on a single-cell basis, followed by purification of cell subsets by sorting into tubes or multiwell formats. The flow cell allows for improved light excitation and collection optics. Fiber optics direct and focus five lasers (360nm, 405nm, 488nm, 561nm, and 633nm) onto the alignment prisms and then focuses on the cuvette flow cell. The FACS Aria II is a high-speed digital sorter that incorporates a fixed-alignment cuvette flow cell. To rapidly retrieve or sort a particular sub-group of cells from a mixed sample, then a cell sorter would be required.įor operator and technical assistance and core staff time, please contact Sarah Elmes at. For more complex assays requiring more than four differentiating markers, then the high-end multi-laser Canto II system would work well. To analyze the light scatter and fluorescence characteristics of a cell population, one of the benchtop machines is appropriate. These systems are primarily used for the rapid analysis and sorting of single-cell suspensions. The LCA provides access and training on two FACS Calibur benchtop cytometers, one FACS Canto II multi-laser digital cytometer, one FACS Aria II cell sorter, one FACS Aria III cell sorter, and one Sony SH800 cell sorter. Flow Cytometry & Cell Sorting Instruments and Services
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